Journal: PLoS ONE

Article Title: Treatment of necrotizing enterocolitis by conditioned medium derived from human amniotic fluid stem cells

doi: 10.1371/journal.pone.0260522

Figure Lengend Snippet: Antibodies used for immunofluorescence of the ileum.

Article Snippet: Primary OLFM4 , Intestinal stem cells , Rabbit , 1:500 , Cell Signalling Technology (Danvers, MS, USA) , 39141.

Techniques: Immunofluorescence, Fluorescence

Journal: PLoS ONE

Article Title: Treatment of necrotizing enterocolitis by conditioned medium derived from human amniotic fluid stem cells

doi: 10.1371/journal.pone.0260522

Figure Lengend Snippet: (A) Survival curves for mice pups from the 3 groups on postnatal days 5 to 9. (B) Representative histological images of the terminal ileum. (C) Histological score of intestinal injury. Inflammatory gene marker expression of (D) Il-6 and (E) Tnf-α . Immunofluorescent protein expression of (F) MPO and (G) CC3 in the terminal ilea of the 3 groups. Yellow arrows indicate positive staining. Relative quantification of the (H) MPO and (I) CC3 protein expressions. (J) Angiogenesis/vascularization Vegf gene expression in the 3 groups. Data are presented as mean ± standard error, with significance of group comparisons based on one-way ANOVA and Tukey post-hoc tests. n = 10 for each group, *p<0.05, **p<0.01, and ***p<0.001.

Article Snippet: Primary OLFM4 , Intestinal stem cells , Rabbit , 1:500 , Cell Signalling Technology (Danvers, MS, USA) , 39141.

Techniques: Marker, Expressing, Staining, Quantitative Proteomics, Gene Expression

Journal: PLoS ONE

Article Title: Treatment of necrotizing enterocolitis by conditioned medium derived from human amniotic fluid stem cells

doi: 10.1371/journal.pone.0260522

Figure Lengend Snippet: NEC induced an intestinal damage, increased inflammation (IL-6 and TNF-α), neutrophil infiltration (MPO), and elevated apoptosis (CC3), as well as decreased angiogenesis or vascularization (VEGF). Further, it diminished epithelial proliferation (Ki67) and stem cell regeneration activity (Lgr5 and Olfm4), however, CM administration reverses all of these effects to yield reduced intestinal injury and improved survival.

Article Snippet: Primary OLFM4 , Intestinal stem cells , Rabbit , 1:500 , Cell Signalling Technology (Danvers, MS, USA) , 39141.

Techniques: Activity Assay

Journal: International Journal of Biological Sciences

Article Title: Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell

doi: 10.7150/ijbs.42331

Figure Lengend Snippet: The expression of Lgr5 in the colon tissue of DSS-induced mice. Immunohistochemical staining of Lgr5 + in colon tissue of (A) WT Slit and Slit2-Tg mice and (B) WT Robo1/2 and Robo1/2 +/- mice (n=6 in each group, scale bar = 20 μm); The mRNA expression of Lgr5 as assessed by RT-qPCR in (C) WT Slit and Slit2-Tg mice and (D) WT Robo1/2 and Robo1/2 +/- mice (n=3 in each group); data are present as means ± SEM. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Therefore, in this study, we explored the role of Slit2/Robo1 in the autophagy of intestinal Lgr5 + stem cells during the development of UC.

Techniques: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR

Journal: International Journal of Biological Sciences

Article Title: Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell

doi: 10.7150/ijbs.42331

Figure Lengend Snippet: Expression of the autophagy proteins in DSS-induced mice. The coexpression of Lgr5 + colonic stem cells and the autophagy protein LC3 in (A) WT Slit and Slit2-Tg mice and (B) WT Robo1/2 and Robo1/2 +/- mice. Lgr5 (green) and LC3 (red) expression in the colonic epithelium. Sections were counterstained with DAPI (blue). (n=5 in each group; scale bar = 50 μm); (C) the photograph of the crypt isolated from colon tissue (scale bar= 200μm); (D) protein expression of LC3II/I in (D) WT Slit and Slit2-Tg mice and (F) WT Robo1/2 and Robo1/2 +/- mice (n=4); The expression of P62 in (E) WT Slit and Slit2-Tg mice and (G) WT Robo1/2 and Robo1/2 +/- mice (n=3-4 in each group). Detection of β-actin served as a loading control. Quantification of bands is expressed as density ratio of indicated protein/β-actin (A.U.); data are present as means ± SEM. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Therefore, in this study, we explored the role of Slit2/Robo1 in the autophagy of intestinal Lgr5 + stem cells during the development of UC.

Techniques: Expressing, Isolation, Control